INFO:

24.3K Views. Icahn School of Medicine at Mount Sinai. The overall goal of this procedure is to generate correctly folded purified influenza, hemagglutinin and neuraminidase surface glycoprotein for immunological assays and vaccination experiments. This is accomplished by first cloning the recombinant BMid carrying influenza genes, followed by generation of recombinant baula viruses. The second step is to use these recombinant baula viruses to express hemagglutinin and neuraminidase proteins in insect cells.Next, the recombinant proteins a...